Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
Identifieur interne : 000194 ( France/Analysis ); précédent : 000193; suivant : 000195Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
Auteurs : Claire Debarnot [France] ; Isabelle Imbert ; François Ferron ; Laure Gluais ; Isabelle Varlet ; Nicolas Papageorgiou ; Mickaël Bouvet ; Julien Lescar ; Etienne Decroly ; Bruno CanardSource :
- Acta crystallographica. Section F, Structural biology and crystallization communications [ 1744-3091 ] ; 2011.
Descripteurs français
- KwdFr :
- Clonage moléculaire, Cristallisation, Cristallographie aux rayons X, Données de séquences moléculaires, Humains, Methyltransferases (), Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (isolement et purification), RNA replicase (), RNA replicase (génétique), RNA replicase (isolement et purification), Virus du SRAS ().
- MESH :
- génétique : Protéines virales non structurales, RNA replicase.
- isolement et purification : Protéines virales non structurales, RNA replicase.
- Clonage moléculaire, Cristallisation, Cristallographie aux rayons X, Données de séquences moléculaires, Humains, Methyltransferases, Protéines virales non structurales, RNA replicase, Virus du SRAS.
English descriptors
- KwdEn :
- Cloning, Molecular, Crystallization, Crystallography, X-Ray, Humans, Methyltransferases (chemistry), Molecular Sequence Data, RNA Replicase (chemistry), RNA Replicase (genetics), RNA Replicase (isolation & purification), SARS Virus (chemistry), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (isolation & purification).
- MESH :
- chemical , chemistry : Methyltransferases, RNA Replicase, Viral Nonstructural Proteins.
- chemical , genetics : RNA Replicase, Viral Nonstructural Proteins.
- chemical , isolation & purification : RNA Replicase, Viral Nonstructural Proteins.
- chemistry : SARS Virus.
- Cloning, Molecular, Crystallization, Crystallography, X-Ray, Humans, Molecular Sequence Data.
Abstract
To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.
Url:
DOI: 10.1107/S1744309111002867
PubMed: 21393853
Affiliations:
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pubmed:21393853Le document en format XML
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last="Debarnot">Claire Debarnot</name><affiliation wicri:level="3"><nlm:affiliation>Département de Virologie Structurale, Architecture et Fonction des Macromolécules Biologiques, UMR 6098, 163 Avenue de Luminy, 13288 Marseille CEDEX 09, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département de Virologie Structurale, Architecture et Fonction des Macromolécules Biologiques, UMR 6098, 163 Avenue de Luminy, 13288 Marseille CEDEX 09</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name></author><author><name sortKey="Gluais, Laure" sort="Gluais, Laure" 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In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><noCountry><name sortKey="Bouvet, Mickael" sort="Bouvet, Mickael" uniqKey="Bouvet M" first="Mickaël" last="Bouvet">Mickaël Bouvet</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name><name sortKey="Lescar, Julien" sort="Lescar, Julien" uniqKey="Lescar J" first="Julien" last="Lescar">Julien Lescar</name><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name><name sortKey="Varlet, Isabelle" sort="Varlet, Isabelle" uniqKey="Varlet I" first="Isabelle" last="Varlet">Isabelle Varlet</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Debarnot, Claire" sort="Debarnot, Claire" uniqKey="Debarnot C" first="Claire" last="Debarnot">Claire Debarnot</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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